Ufarte_2017_PLoS.One_12_e0189201

Reference

Title : Discovery of carbamate degrading enzymes by functional metagenomics - Ufarte_2017_PLoS.One_12_e0189201
Author(s) : Ufarte L , Laville E , Duquesne S , Morgavi D , Robe P , Klopp C , Rizzo A , Pizzut-Serin S , Potocki-Veronese G
Ref : PLoS ONE , 12 :e0189201 , 2017
Abstract :

Bioremediation of pollutants is a major concern worldwide, leading to the research of new processes to break down and recycle xenobiotics and environment contaminating polymers. Among them, carbamates have a very broad spectrum of uses, such as toxinogenic pesticides or elastomers. In this study, we mined the bovine rumen microbiome for carbamate degrading enzymes. We isolated 26 hit clones exhibiting esterase activity, and were able to degrade at least one of the targeted polyurethane and pesticide carbamate compounds. The most active clone was deeply characterized. In addition to Impranil, this clone was active on Tween 20, pNP-acetate, butyrate and palmitate, and on the insecticide fenobucarb. Sequencing and sub-cloning of the best target revealed a novel carboxyl-ester hydrolase belonging to the lipolytic family IV, named CE_Ubrb. This study highlights the potential of highly diverse microbiota such as the ruminal one for the discovery of promiscuous enzymes, whose versatility could be exploited for industrial uses.

PubMedSearch : Ufarte_2017_PLoS.One_12_e0189201
PubMedID: 29240834
Gene_locus related to this paper: 9bact-CEUbrb

Related information

Substrate Impranil    Fenobucarb
Gene_locus Impranil    Fenobucarb    9bact-CEUbrb

Citations formats

Ufarte L, Laville E, Duquesne S, Morgavi D, Robe P, Klopp C, Rizzo A, Pizzut-Serin S, Potocki-Veronese G (2017)
Discovery of carbamate degrading enzymes by functional metagenomics
PLoS ONE 12 :e0189201

Ufarte L, Laville E, Duquesne S, Morgavi D, Robe P, Klopp C, Rizzo A, Pizzut-Serin S, Potocki-Veronese G (2017)
PLoS ONE 12 :e0189201