| Title : Phytochemical Constituents and Toxicity of Hydroalcoholic Extract in - Valeria_2014_Evid.Based.Complement.Alternat.Med_2014_838101 |
| Author(s) : Valeria Soares de Araujo Pinho F , Felipe da Silva G , Echeverria Macedo G , Raquel Muller K , Kemmerich Martins I , Lausmann Ternes AP , Martins da Costa JG , Athayde ML , Boligon AA , Kamdem JP , Franco JL , Rose Alencar de Menezes I , Posser T |
| Ref : Evid Based Complement Alternat Med , 2014 :838101 , 2014 |
| Abstract : Duguetia furfuracea is frequently used as a medicinal plant in Brazil. However, studies have evidenced its cytotoxic, bactericide, and antitumor activities. In the present study we aimed to evaluate the potential toxicity of hydroalcoholic leaves extracts of D. furfuracea (HEDF) in a Drosophila melanogaster model. Toxicity was assessed as changes in locomotor performance, mitochondrial activity, oxidative stress, MAPKs phosphorylation, and apoptosis induction after exposure to HEDF concentrations (1-50 mg/mL) for 7 days. The phytoconstituents of the plant were screened for the presence of alkaloids, tannins, xanthones, chalcones, flavonoids, aurones, and phenolic acids. Exposure of adult flies to HEDF caused mitochondrial dysfunction, overproduction of ROS, and alterations in the activity of detoxifying enzymes GST, SOD and CAT. Induction of ERK phosphorylation and PARP cleavage was also observed, indicating occurrence of HEDF-induced cell stress and apoptotic cell death. In parallel, alterations in cholinesterase activity and impairments in negative geotaxis behavior were observed. Our study draws attention to the indiscriminate use of this plant by population and suggests oxidative stress as a major mechanism underlying its toxicity. |
| ESTHER : Valeria_2014_Evid.Based.Complement.Alternat.Med_2014_838101 |
| PubMedSearch : Valeria_2014_Evid.Based.Complement.Alternat.Med_2014_838101 |
| PubMedID: 25435894 |
Valeria Soares de Araujo Pinho F, Felipe da Silva G, Echeverria Macedo G, Raquel Muller K, Kemmerich Martins I, Lausmann Ternes AP, Martins da Costa JG, Athayde ML, Boligon AA, Kamdem JP, Franco JL, Rose Alencar de Menezes I, Posser T (2014)
Phytochemical Constituents and Toxicity of Hydroalcoholic Extract in
Evid Based Complement Alternat Med
2014 :838101
Valeria Soares de Araujo Pinho F, Felipe da Silva G, Echeverria Macedo G, Raquel Muller K, Kemmerich Martins I, Lausmann Ternes AP, Martins da Costa JG, Athayde ML, Boligon AA, Kamdem JP, Franco JL, Rose Alencar de Menezes I, Posser T (2014)
Evid Based Complement Alternat Med
2014 :838101