| Title : ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae - Vishnu_2015_FEBS.Lett_589_23 |
| Author(s) : Vishnu Varthini L , Selvaraju K , Srinivasan M , Nachiappan V |
| Ref : FEBS Letters , 589 :23 , 2015 |
|
Abstract :
Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deletion of ROG1 and monoglyceride lipase YJU3 (yju3deltarog1delta) and by site-directed mutagenesis of the ROG1 lipase motif (GXSXG). Rog1p is localized in both the cytosol and the nucleus. Overexpression of ROG1 in a ROG1-deficient strain resulted in an accumulation of reactive oxygen species. These results suggest that Rog1p is a MAG lipase that regulates lipid homeostasis. |
| PubMedSearch : Vishnu_2015_FEBS.Lett_589_23 |
| PubMedID: 25433290 |
| Gene_locus related to this paper: yeast-ROG1 |
| Gene_locus | yeast-ROG1 |
Vishnu Varthini L, Selvaraju K, Srinivasan M, Nachiappan V (2015)
ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae
FEBS Letters
589 :23
Vishnu Varthini L, Selvaraju K, Srinivasan M, Nachiappan V (2015)
FEBS Letters
589 :23