| Title : Munc18-1 promotes large dense-core vesicle docking - Voets_2001_Neuron_31_581 |
| Author(s) : Voets T , Toonen RF , Brian EC , de Wit H , Moser T , Rettig J , Sudhof TC , Neher E , Verhage M |
| Ref : Neuron , 31 :581 , 2001 |
|
Abstract :
Secretory vesicles dock at the plasma membrane before Ca(2+) triggers their exocytosis. Exocytosis requires the assembly of SNARE complexes formed by the vesicle protein Synaptobrevin and the membrane proteins Syntaxin-1 and SNAP-25. We analyzed the role of Munc18-1, a cytosolic binding partner of Syntaxin-1, in large dense-core vesicle (LDCV) secretion. Calcium-dependent LDCV exocytosis was reduced 10-fold in mouse chromaffin cells lacking Munc18-1, but the kinetic properties of the remaining release, including single fusion events, were not different from controls. Concomitantly, mutant cells displayed a 10-fold reduction in morphologically docked LDCVs. Moreover, acute overexpression of Munc18-1 in bovine chromaffin cells increased the amount of releasable vesicles and accelerated vesicle supply. We conclude that Munc18-1 functions upstream of SNARE complex formation and promotes LDCV docking. |
| PubMedSearch : Voets_2001_Neuron_31_581 |
| PubMedID: 11545717 |
Voets T, Toonen RF, Brian EC, de Wit H, Moser T, Rettig J, Sudhof TC, Neher E, Verhage M (2001)
Munc18-1 promotes large dense-core vesicle docking
Neuron
31 :581
Voets T, Toonen RF, Brian EC, de Wit H, Moser T, Rettig J, Sudhof TC, Neher E, Verhage M (2001)
Neuron
31 :581