Vonk_2013_Proc.Natl.Acad.Sci.U.S.A_110_20651

Reference

Title : The king cobra genome reveals dynamic gene evolution and adaptation in the snake venom system - Vonk_2013_Proc.Natl.Acad.Sci.U.S.A_110_20651
Author(s) : Vonk FJ , Casewell NR , Henkel CV , Heimberg AM , Jansen HJ , McCleary RJ , Kerkkamp HM , Vos RA , Guerreiro I , Calvete JJ , Wuster W , Woods AE , Logan JM , Harrison RA , Castoe TA , de Koning AP , Pollock DD , Yandell M , Calderon D , Renjifo C , Currier RB , Salgado D , Pla D , Sanz L , Hyder AS , Ribeiro JM , Arntzen JW , van den Thillart GE , Boetzer M , Pirovano W , Dirks RP , Spaink HP , Duboule D , McGlinn E , Kini RM , Richardson MK
Ref : Proc Natl Acad Sci U S A , 110 :20651 , 2013
Abstract :

Snakes are limbless predators, and many species use venom to help overpower relatively large, agile prey. Snake venoms are complex protein mixtures encoded by several multilocus gene families that function synergistically to cause incapacitation. To examine venom evolution, we sequenced and interrogated the genome of a venomous snake, the king cobra (Ophiophagus hannah), and compared it, together with our unique transcriptome, microRNA, and proteome datasets from this species, with data from other vertebrates. In contrast to the platypus, the only other venomous vertebrate with a sequenced genome, we find that snake toxin genes evolve through several distinct co-option mechanisms and exhibit surprisingly variable levels of gene duplication and directional selection that correlate with their functional importance in prey capture. The enigmatic accessory venom gland shows a very different pattern of toxin gene expression from the main venom gland and seems to have recruited toxin-like lectin genes repeatedly for new nontoxic functions. In addition, tissue-specific microRNA analyses suggested the co-option of core genetic regulatory components of the venom secretory system from a pancreatic origin. Although the king cobra is limbless, we recovered coding sequences for all Hox genes involved in amniote limb development, with the exception of Hoxd12. Our results provide a unique view of the origin and evolution of snake venom and reveal multiple genome-level adaptive responses to natural selection in this complex biological weapon system. More generally, they provide insight into mechanisms of protein evolution under strong selection.

PubMedSearch : Vonk_2013_Proc.Natl.Acad.Sci.U.S.A_110_20651
PubMedID: 24297900
Gene_locus related to this paper: ophha-v8p7p1.1 , ophha-v8p7p1.3 , ophha-v8n6c3 , ophha-v8p9v2 , ophha-v8p8q1 , ophha-v8p760 , ophha-v8pg65 , ophha-v8pgg0 , ophha-v8p9z4.2 , ophha-v8nra3 , ophha-v8p430 , ophha-v8pbf0.2

Related information

Gene_locus ophha-v8p7p1.1    ophha-v8p7p1.3    ophha-v8n6c3    ophha-v8p9v2    ophha-v8p8q1    ophha-v8p760    ophha-v8pg65    ophha-v8pgg0    ophha-v8p9z4.2    ophha-v8nra3    ophha-v8p430    ophha-v8pbf0.2
Gene_locus_frgt ophha-v8p7p1.2    ophha-v8n5s8    ophha-v8npz5    ophha-v8nfj8    ophha-v8p3m0    ophha-v8nnw7    ophha-v8n2l5    ophha-v8nzn6    ophha-v8ndi3    ophha-v8ncs1    ophha-v8nwe4    ophha-v8n3n0    ophha-v8nsf3    ophha-v8nr78    ophha-v8nbb1    ophha-v8n0n2    ophha-v8n2k1    ophha-v8n869    ophha-v8n4u3    ophha-v8nt01    ophha-v8p728    ophha-v8p8z8    ophha-v8p9z4.1    ophha-v8n8q6    ophha-v8n892    ophha-v8pbf0.1

Citations formats

Vonk FJ, Casewell NR, Henkel CV, Heimberg AM, Jansen HJ, McCleary RJ, Kerkkamp HM, Vos RA, Guerreiro I, Calvete JJ, Wuster W, Woods AE, Logan JM, Harrison RA, Castoe TA, de Koning AP, Pollock DD, Yandell M, Calderon D, Renjifo C, Currier RB, Salgado D, Pla D, Sanz L, Hyder AS, Ribeiro JM, Arntzen JW, van den Thillart GE, Boetzer M, Pirovano W, Dirks RP, Spaink HP, Duboule D, McGlinn E, Kini RM, Richardson MK (2013)
The king cobra genome reveals dynamic gene evolution and adaptation in the snake venom system
Proc Natl Acad Sci U S A 110 :20651

Vonk FJ, Casewell NR, Henkel CV, Heimberg AM, Jansen HJ, McCleary RJ, Kerkkamp HM, Vos RA, Guerreiro I, Calvete JJ, Wuster W, Woods AE, Logan JM, Harrison RA, Castoe TA, de Koning AP, Pollock DD, Yandell M, Calderon D, Renjifo C, Currier RB, Salgado D, Pla D, Sanz L, Hyder AS, Ribeiro JM, Arntzen JW, van den Thillart GE, Boetzer M, Pirovano W, Dirks RP, Spaink HP, Duboule D, McGlinn E, Kini RM, Richardson MK (2013)
Proc Natl Acad Sci U S A 110 :20651