Title : A splicing variant of EDS1 from Vitis vinifera forms homodimers but no heterodimers with PAD4 - Voss_2023_Protein.Sci__e4624
Author(s) : Voss M , Cseke LJ , Gassmann W , Niefind K
Ref : Protein Science , :e4624 , 2023
Abstract :

Enhanced Disease Susceptibility 1 (EDS1), a key component of microbe-triggered immunity (MTI) and effector-triggered immunity (ETI) in most higher plants, forms functional heterodimeric complexes with its homologs Phytoalexin-Deficient 4 (PAD4) or Senescence-Associated Gene 101 (SAG101). Here, the crystal structure of VvEDS1(Nterm) , the N-terminal domain of EDS1 from Vitis vinifera, is reported, representing the first structure of an EDS1 entity beyond the model plant Arabidopsis thaliana. VvEDS1(Nterm) has an alpha/beta-hydrolase fold, is similar to the N-terminal domain of A. thaliana EDS1 and forms stable homodimers in solution as well as in crystals. These VvEDS1(Nterm) homodimers are spatially incompatible with heterodimers with PAD4 or SAG101, they explain why VvEDS1(Nterm) does not interact with Vitis vinifera PAD4 according to gel filtration, and they serve as a guide to develop a plausible, albeit experimentally not verified model of full-length VvEDS1. VvEDS1(Nterm) is a splicing variant comprising two of three exons of the VvEDS1 gene. It originates from a naturally occurring mRNA, in which the first of two introns was removed while the second one containing a stop codon close to the exon/intron border was retained. This is a potential case of intron retention and the first report of this phenomenon in the context of EDS1. Its biological significance has not yet been clarified, nor has the question if a VvEDS1(Nterm) protein with a specific function can occur under physiological conditions. This article is protected by copyright. All rights reserved.

PubMedSearch : Voss_2023_Protein.Sci__e4624
PubMedID: 36917448
Gene_locus related to this paper: vitvi-f6gsx7

Related information

Gene_locus vitvi-f6gsx7
Structure vitvi-f6gsx7    8B28

Citations formats

Voss M, Cseke LJ, Gassmann W, Niefind K (2023)
A splicing variant of EDS1 from Vitis vinifera forms homodimers but no heterodimers with PAD4
Protein Science :e4624

Voss M, Cseke LJ, Gassmann W, Niefind K (2023)
Protein Science :e4624