Title : Enzyme-octadecylamine Langmuir-Blodgett membranes for ENFET biosensors - Wan_2000_Talanta_52_663 |
Author(s) : Wan K , Chovelon JM , Jaffrezic-Renault N |
Ref : Talanta , 52 :663 , 2000 |
Abstract :
Langmuir-Blodgett (LB) films containing butyrylcholinesterase (BuChE) are fabricated to realise an enzymatic field effect transistor (ENFET) for the detection of organophosphorus pesticides in water. Trichlorfon as a common pesticide is examined in our work. The BuChE-immobilised LB films are formed by adsorbing the enzyme molecules onto a stearylamine monolayer using the electrostatic force. Enzyme/stearylamine mixed LB films are immobilized onto a pH-ISFET surface and treated by glutaraldehyde vapour to improve the LB film's stability. The ENFET thus obtained worked as a potentiometric biosensor for trichlorfon detection on the basis of enzyme inhibition. The detection limit for trichlorfon can reach 10(-7) M (26 ppb). The surface characteristics of BuChE/stearylamine LB films obtained under various conditions of the dipping surface pressures are analysed qualitatively by atomic force microscopy (AFM) and analysed quantitatively by FTIR spectroscopy. |
PubMedSearch : Wan_2000_Talanta_52_663 |
PubMedID: 18968024 |
Wan K, Chovelon JM, Jaffrezic-Renault N (2000)
Enzyme-octadecylamine Langmuir-Blodgett membranes for ENFET biosensors
Talanta
52 :663
Wan K, Chovelon JM, Jaffrezic-Renault N (2000)
Talanta
52 :663