Title : Crystallization and preliminary crystallographic analysis of acylamino-acid releasing enzyme from the hyperthermophilic archaeon Aeropyrum pernix - Wang_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1054 |
Author(s) : Wang G , Gao R , Ding Y , Yang H , Cao S , Feng Y , Rao Z |
Ref : Acta Crystallographica D Biol Crystallogr , 58 :1054 , 2002 |
Abstract :
Crystals of acylamino-acid releasing enzyme from the hyperthermophilic archaeon Aeropyrum pernix strain K1 have been grown at 291 K using ammonium phosphate as a precipitant. The diffraction pattern of the crystal extends to 2.4 A resolution at 100 K using Cu Kalpha radiation. The crystal belongs to space group P1, with unit-cell parameters a = 107.5, b = 109.9, c = 119.4 A, alpha = 108.1, beta = 109.8, gamma = 91.9 degrees. The presence of eight molecules per asymmetric unit gives a crystal volume per protein mass (V(M)) of 2.4 A(3) Da(-1) and a solvent content of 48% by volume. A full set of X-ray diffraction data was collected to 2.9 A from the native crystal. |
PubMedSearch : Wang_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1054 |
PubMedID: 12037315 |
Gene_locus related to this paper: aerpe-APE1547 |
Substrate | P-nitrophenyl-phosphate |
Gene_locus | aerpe-APE1547 |
Structure | 1VE6 1VE7 |
Wang G, Gao R, Ding Y, Yang H, Cao S, Feng Y, Rao Z (2002)
Crystallization and preliminary crystallographic analysis of acylamino-acid releasing enzyme from the hyperthermophilic archaeon Aeropyrum pernix
Acta Crystallographica D Biol Crystallogr
58 :1054
Wang G, Gao R, Ding Y, Yang H, Cao S, Feng Y, Rao Z (2002)
Acta Crystallographica D Biol Crystallogr
58 :1054