Wang_2010_Appl.Environ.Microbiol_76_2524

Reference

Title : AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum - Wang_2010_Appl.Environ.Microbiol_76_2524
Author(s) : Wang WZ , Morohoshi T , Ikenoya M , Someya N , Ikeda T
Ref : Applied Environmental Microbiology , 76 :2524 , 2010
Abstract :

N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signal molecules by many Gram-negative bacteria. We have reported that Microbacterium testaceum StLB037, which was isolated from the leaf surface of potato, has AHL-degrading activity. In this study, we cloned the aiiM gene from the genomic library of StLB037, which has AHL-degrading activity and shows high homology with the alpha/beta hydrolase fold family from Actinobacteria. Purified AiiM as a maltose binding fusion protein showed high degrading activity of AHLs with both short- and long-chain AHLs with or without substitution at carbon 3. High-performance liquid chromatography analysis revealed that AiiM works as an AHL lactonase that catalyzes AHL ring opening by hydrolyzing lactones. In addition, expression of AiiM in the plant pathogen Pectobacterium carotovorum subsp. carotovorum reduced pectinase activity markedly and attenuated soft rot symptoms on potato slices. In conclusion, this study indicated that AiiM might be effective in quenching quorum sensing of P. carotovorum subsp. carotovorum.

PubMedSearch : Wang_2010_Appl.Environ.Microbiol_76_2524
PubMedID: 20173075
Gene_locus related to this paper: micts-ahll

Related information

Gene_locus micts-ahll

Citations formats

Wang WZ, Morohoshi T, Ikenoya M, Someya N, Ikeda T (2010)
AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum
Applied Environmental Microbiology 76 :2524

Wang WZ, Morohoshi T, Ikenoya M, Someya N, Ikeda T (2010)
Applied Environmental Microbiology 76 :2524