Wang_2011_Analyst_136_3349

Reference

Title : TiO2-decorated graphene nanohybrids for fabricating an amperometric acetylcholinesterase biosensor - Wang_2011_Analyst_136_3349
Author(s) : Wang K , Li HN , Wu J , Ju C , Yan JJ , Liu Q , Qiu B
Ref : Analyst , 136 :3349 , 2011
Abstract :

This work describes a highly sensitive and rapid amperometric biosensor for organophosphate compounds (OPs) based on immobilization of acetylcholinesterase (AChE) on a novel TiO(2)-decorated graphene (TiO(2)-G) nanohybrid, which was constructed by in situ growth of TiO(2) nanoparticles (NPs) on the graphene sheet. The well-dispersed TiO(2) NPs eliminated the restacking of TiO(2)-G nanohybrids. Due to the integrating of TiO(2)-G nanohybrids, the as-prepared biosensor showed high affinity to acetylthiocholine (ATCl) with a Michaelis-Menten constant (K(m)) value of 0.22 mM, and rapid inhibition time (3 min). Further, based on the inhibition of OPs on the enzymatic activity of the immobilized AChE, and using carbaryl as a model compound, the inhibition of carbaryl was proportional to its concentration ranging from 0.001 to 0.015 and 0.015 to 2 mug mL(-1) with a detection limit of 0.3 ng mL(-1) (S/N = 3). The developed biosensor exhibited a good performance for organophosphate pesticide detection, including good reproducibility and acceptable stability, which provided a new and promising tool for the analysis of enzyme inhibitors.

PubMedSearch : Wang_2011_Analyst_136_3349
PubMedID: 21738917

Related information

Citations formats

Wang K, Li HN, Wu J, Ju C, Yan JJ, Liu Q, Qiu B (2011)
TiO2-decorated graphene nanohybrids for fabricating an amperometric acetylcholinesterase biosensor
Analyst 136 :3349

Wang K, Li HN, Wu J, Ju C, Yan JJ, Liu Q, Qiu B (2011)
Analyst 136 :3349