| Title : Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells - Wang_2013_Evid.Based.Complement.Alternat.Med_2013_937370 |
| Author(s) : Wang Y , Pan WL , Liang WC , Law WK , Tsz-Ming Ip D , Ng TB , Miu-Yee Waye M , Wan DC |
| Ref : Evid Based Complement Alternat Med , 2013 :937370 , 2013 |
| Abstract : Acetylcholinesterase inhibitors are prominent alternative in current clinical treatment for AD patients. Therefore, there is a continued need to search for novel AChEIs with good clinical efficacy and less side effects. By using our in-house natural product database and AutoDock Vina as a tool in docking study, we have identified twelve phytochemicals (emodin, aloe-emodin, chrysophanol, and rhein in Rhei Radix Et Rhizoma; xanthotoxin, phellopterin, alloisoimperatorin, and imperatorin in Angelicae dahuricae Radix; shikonin, acetylshikonin, isovalerylshikonin, and beta,beta-dimethylacrylshikonin in Arnebiae Radix) as candidates of AChEIs that were not previously reported in the literature. In addition to AChEI activity, a series of cell-based experiments were conducted for the investigation of their neuroprotective activities. We found that acetylshikonin and its derivatives prevented apoptotic cell death induced by hydrogen peroxide in human and rat neuronal SH-SY5Y and PC12 cells at 10 muM. We showed that acetylshikonin exhibited the most potent antiapoptosis activity through the inhibition of the generation of reactive oxygen species as well as protection of the loss of mitochondria membrane potential. Furthermore, we identified for the first time that the upregulation of heme oxygenase 1 by acetylshikonin is a key step mediating its antiapoptotic activity from oxidative stress in SH-SY5Y cells. |
| ESTHER : Wang_2013_Evid.Based.Complement.Alternat.Med_2013_937370 |
| PubMedSearch : Wang_2013_Evid.Based.Complement.Alternat.Med_2013_937370 |
| PubMedID: 24302971 |
Wang Y, Pan WL, Liang WC, Law WK, Tsz-Ming Ip D, Ng TB, Miu-Yee Waye M, Wan DC (2013)
Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells
Evid Based Complement Alternat Med
2013 :937370
Wang Y, Pan WL, Liang WC, Law WK, Tsz-Ming Ip D, Ng TB, Miu-Yee Waye M, Wan DC (2013)
Evid Based Complement Alternat Med
2013 :937370