Wang_2015_World.J.Gastroenterol_21_7172

Reference

Title : Abundance and significance of neuroligin-1 and glutamate in Hirschsprung's disease - Wang_2015_World.J.Gastroenterol_21_7172
Author(s) : Wang J , Du H , Mou YR , Niu JY , Zhang WT , Yang HC , Li AW
Ref : World J Gastroenterol , 21 :7172 , 2015
Abstract :

AIM: To investigate the abundance and potential diagnostic significance of neuroligin-1 and glutamate (Glu) in Hirschsprung's disease (HSCR).
METHODS: Ninety children with HSCR and 50 children without HSCR matched for similar nutritional status, age and basal metabolic index were studied. The expression and localization of neuroligin-1 and Glu were assessed using double-labeling immunofluorescence staining of longitudinal muscles with adherent myenteric plexus from the surgically excised colon of children with HSCR. Western blot analysis, quantitative real-time PCR (qRT-PCR) and immunohistochemistry were performed to evaluate the abundance of neuroligin-1 and Glu in different HSCR-affected segments (ganglionic, transitional, and aganglionic segments). Enzyme-linked immunosorbent assay (ELISA) was used to detect and compare serum Glu levels in the long-segment HSCR, short-segment HSCR and non-HSCR samples.
RESULTS: Neuroligin-1 and Glu were co-expressed highest to lowest in the ganglionic, transitional and aganglionic segments based on Western blot (neuroligin-1: 0.177 +/- 0.008 vs 0.101 +/- 0.006, 0.177 +/- 0.008 vs 0.035 +/- 0.005, and 0.101 +/- 0.006 vs 0.035 +/- 0.005, P < 0.005; Glu: 0.198 +/- 0.006 vs 0.115 +/- 0.008, 0.198 +/- 0.006 vs 0.040 +/- 0.003, and 0.115 +/- 0.008 vs 0.040 +/- 0.003, P < 0.005) and qRT-PCR (neuroligin-1: 9.58 x 10(-5) +/- 9.94 x 10(-6) vs 2.49 x 10(-5) +/- 1.38 x 10(-6), 9.58 x 10(-5) +/- 9.94 x 10(-6) vs 7.17 x 10(-6 +/-) 1.12 x 10(-6), and 2.49 x 10(-5) +/- 1.38 x 10(-6) vs 7.17 x 10(-6) +/- 1.12 x 10(-6), P < 0.005). Serum Glu level was the highest to lowest in the non-HSCR, short-type HSCR and long-type HSCR samples based on ELISA (in nmol/muL, 0.93 +/- 0.31 vs 0.57 +/- 0.25, 0.93 +/- 0.31 vs 0.23 +/- 0.16, and 0.57 +/- 0.25 vs 0.23 +/- 0.16, P < 0.005). CONCLUSION: Neuroligin-1 and Glu may represent new markers of ganglion cells, whose expression may correlate with the pathogenesis, diagnosis, differential diagnosis or classification of HSCR.

PubMedSearch : Wang_2015_World.J.Gastroenterol_21_7172
PubMedID: 26109803

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Citations formats

Wang J, Du H, Mou YR, Niu JY, Zhang WT, Yang HC, Li AW (2015)
Abundance and significance of neuroligin-1 and glutamate in Hirschsprung's disease
World J Gastroenterol 21 :7172

Wang J, Du H, Mou YR, Niu JY, Zhang WT, Yang HC, Li AW (2015)
World J Gastroenterol 21 :7172