Title : The endocannabinoid system regulates synaptic transmission in nucleus accumbens by increasing DAGL-alpha expression following short-term morphine withdrawal - Wang_2016_Br.J.Pharmacol_173_1143 |
Author(s) : Wang XQ , Ma J , Cui W , Yuan WX , Zhu G , Yang Q , Heng LJ , Gao GD |
Ref : British Journal of Pharmacology , 173 :1143 , 2016 |
Abstract :
BACKGROUND AND PURPOSE: The endocannabinoid (eCB) system is involved in pathways that regulate drug addiction and eCB-mediated synaptic plasticity has been linked with addictive behaviours. Here, we investigated the molecular mechanisms underlying the changes in eCB-dependent synaptic plasticity in the nucleus accumbens core (NAcc) following short-term withdrawal from repeated morphine treatment. EXPERIMENTAL APPROACH: Conditioned place preference (CPP) was used to evaluate the rewarding effects of morphine in rats. Evoked inhibitory postsynaptic currents of medium spiny neurons in NAcc were measured using whole-cell patch-clamp recordings. Changes in depolarization-induced suppression of inhibition (DSI) in the NAcc were assessed to determine the effect of short-term morphine withdrawal on the eCB system. To identify the potential modulation mechanism of short-term morphine withdrawal on the eCB system, the expression of diacylglycerol lipase alpha (DGL-alpha) and monoacylglycerol lipase was detected by Western blot analysis. KEY |
PubMedSearch : Wang_2016_Br.J.Pharmacol_173_1143 |
PubMedID: 25296881 |
Wang XQ, Ma J, Cui W, Yuan WX, Zhu G, Yang Q, Heng LJ, Gao GD (2016)
The endocannabinoid system regulates synaptic transmission in nucleus accumbens by increasing DAGL-alpha expression following short-term morphine withdrawal
British Journal of Pharmacology
173 :1143
Wang XQ, Ma J, Cui W, Yuan WX, Zhu G, Yang Q, Heng LJ, Gao GD (2016)
British Journal of Pharmacology
173 :1143