| Title : Diterpenoids from the roots of Euphorbia ebracteolata and their inhibitory effects on human carboxylesterase 2 - Wang_2017_Phytochemistry_146_82 |
| Author(s) : Wang AH , Tian XG , Cui YL , Huo XK , Zhang BJ , Deng S , Feng L , Ma XC , Jia JM , Wang C |
| Ref : Phytochemistry , 146 :82 , 2017 |
|
Abstract :
A chemical investigation of the roots of Euphorbia ebracteolata identified eighteen diterpenoids and glycosides. On the basis of spectroscopic data, they were determined to be ent-kauranes, ent-atisanes, tigliane derivatives, ingenane, and ent-abietanes, among which were eleven previously undescribed diterpenoids. The inhibitory effects of the isolated compounds against human carboxylesterase 2 (hCE-2) were evaluated in vitro, which revealed moderate inhibitory effects with IC50 values<50muM. Next, the inhibitory kinetics were evaluated for the putative hCE-2 inhibitor 4beta,9alpha,16,20-tetrahydroxy-14(13-->12)-abeo-12alphaH-1,6-tigliadiene-3,13-di one (IC50 3.88muM), and results indicated competitive inhibition with Ki 4.94muM. Additionally, none of the diterpenoids showed cytotoxic effects against five human tumor cell lines as determined by MTT assays. |
| PubMedSearch : Wang_2017_Phytochemistry_146_82 |
| PubMedID: 29253734 |
Wang AH, Tian XG, Cui YL, Huo XK, Zhang BJ, Deng S, Feng L, Ma XC, Jia JM, Wang C (2017)
Diterpenoids from the roots of Euphorbia ebracteolata and their inhibitory effects on human carboxylesterase 2
Phytochemistry
146 :82
Wang AH, Tian XG, Cui YL, Huo XK, Zhang BJ, Deng S, Feng L, Ma XC, Jia JM, Wang C (2017)
Phytochemistry
146 :82