Wang_2018_Int.J.Syst.Evol.Microbiol_68_1896

A yellow-pigmented bacterial strain (JR1(T)) isolated from a sediment sample was subjected to a taxonomic study, based on phenotypic, genetic and physiological characterization. Here, we describe the cultivation and characteristics of strain JR1(T), a novel member of the genus Carboxylicivirga in the family Marinilabiliaceae. Cells of strain JR1(T) were rod-shaped, Gram-stain-negative, non-motile and facultatively anaerobic. The temperature range for growth was 15-42 degreesC (optimum, 33 degreesC) and the pH range for growth was pH 6.0-8.5 (optimum, pH 7.0-7.5). Growth occurred in the presence of 0.0-10.0 % (w/v) NaCl (optimum 2.0-3.0 %). 16S rRNA gene sequence analysis produced results with 97.4 % similarity to Carboxylicivirga taeanensisMEBiC 08903(T), 96.8 % similarity to Carboxylicivirga mesophilaMEBiC 07026(T), 94.9 % similarity to Carboxylicivirga linearis FB218(T) and 94.6 % similarity to Carboxylicivirga flava Q15(T). The DNA G+C content was 42.3 mol% and the major fatty acids were iso-C15 : 0, C15 : 0, anteiso-C15 : 0, C17 : 1omega6c and iso-C17 : 0-3OH. The major polar lipids detected were phosphatidylethanolamine and two unidentified lipids; the major respiratory quinone detected was MK-7. The results of the phenotypical, phylogenetic and biochemical analyses between the study strain and some related type strains indicated that this strain represent a novel species of the genus Carboxylicivirga within the family Marinilabiliaceae, for which the name Carboxylicivirga sediminis sp. nov. is proposed. The type strain is JR1(T) (=MCCC 1K03323(T)=KCTC 52869(T)).