| Title : Structural insight into the carboxylesterase BioH from Klebsiella pneumoniae - Wang_2019_Biochem.Biophys.Res.Commun_520_538 |
| Author(s) : Wang L , Chen Y , Shang F , Liu W , Lan J , Gao P , Ha NC , Nam KH , Dong Y , Quan C , Xu Y |
| Ref : Biochemical & Biophysical Research Communications , 520 :538 , 2019 |
| Abstract : The BioH carboxylesterase which is a typical alpha/beta-hydrolase enzyme involved in biotin synthetic pathway in most bacteria. BioH acts as a gatekeeper and blocks the further elongation of its substrate. In the pathogen Klebsiella pneumoniae, BioH plays a critical role in the biosynthesis of biotin. To better understand the molecular function of BioH, we determined the crystal structure of BioH from K. pneumoniae at 2.26A resolution using X-ray crystallography. The structure of KpBioH consists of an alpha-beta-alpha sandwich domain and a cap domain. B-factor analysis revealed that the alpha-beta-alpha sandwich domain is a rigid structure, while the loops in the cap domain shows the structural flexibility. The active site of KpBioH contains the catalytic triad (Ser82-Asp207-His235) on the interface of the alpha-beta-alpha sandwich domain, which is surrounded by the cap domain. Size exclusion chromatography shows that KpBioH prefers the monomeric state in solution, whereas two-fold symmetric dimeric formation of KpBioH was observed in the asymmetric unit, the conserved Cys31-based disulfide bonds can maintain the irreversible dimeric formation of KpBioH. Our study provides important structural insight for understanding the molecular mechanisms of KpBioH and its homologous proteins. |
| ESTHER : Wang_2019_Biochem.Biophys.Res.Commun_520_538 |
| PubMedSearch : Wang_2019_Biochem.Biophys.Res.Commun_520_538 |
| PubMedID: 31615653 |
| Gene_locus related to this paper: klep3-bioh |
| Gene_locus related to this paper: klep3-bioh |
Wang L, Chen Y, Shang F, Liu W, Lan J, Gao P, Ha NC, Nam KH, Dong Y, Quan C, Xu Y (2019)
Structural insight into the carboxylesterase BioH from Klebsiella pneumoniae
Biochemical & Biophysical Research Communications
520 :538
Wang L, Chen Y, Shang F, Liu W, Lan J, Gao P, Ha NC, Nam KH, Dong Y, Quan C, Xu Y (2019)
Biochemical & Biophysical Research Communications
520 :538