Wang_2021_Acta.Crystallogr.D.Struct.Biol_77_1554

Reference

Title : Structural and biochemical analyses of the tetrameric carboxypeptidase S9Cfn from Fusobacterium nucleatum - Wang_2021_Acta.Crystallogr.D.Struct.Biol_77_1554
Author(s) : Wang X , Cheng MT , Chen ZP , Jiang YL , Ge YS , Xia R , Hou WT
Ref : Acta Crystallographica D Struct Biol , 77 :1554 , 2021
Abstract :

As one of the most abundant bacteria in the human oral cavity, Fusobacterium nucleatum is closely involved in various oral diseases and is also a risk factor for other diseases. The peptidases of F. nucleatum can digest exogenous peptides into amino acids to satisfy its nutrient requirements. Here, a putative F. nucleatum peptidase, termed S9Cfn, which belongs to the S9C peptidase family was identified. Enzymatic activity assays combined with mass-spectrometric analysis revealed that S9Cfn is a carboxypeptidase, but not an aminopeptidase as previously annotated. The crystal structure of the S9Cfn tetramer was solved at 2.6A resolution and was found to contain a pair of oligomeric pores in the center. Structural analysis, together with site-directed mutagenesis and enzymatic activity assays, revealed a substrate-entrance tunnel that extends from each oligomeric pore to the catalytic triad, adjacent to which three conserved arginine residues are responsible for substrate binding. Moreover, comparison with other S9 peptidase structures indicated drastic conformational changes of the oligomeric pores during the catalytic cycle. Together, these findings increase the knowledge of this unique type of tetrameric carboxypeptidase and provide insight into the homeostatic control of microbiota in the human oral cavity.

PubMedSearch : Wang_2021_Acta.Crystallogr.D.Struct.Biol_77_1554
PubMedID: 34866611
Gene_locus related to this paper: fusnu-a0a1s1mbe1

Related information

Gene_locus fusnu-a0a1s1mbe1
Structure fusnu-a0a1s1mbe1    7EP9

Citations formats

Wang X, Cheng MT, Chen ZP, Jiang YL, Ge YS, Xia R, Hou WT (2021)
Structural and biochemical analyses of the tetrameric carboxypeptidase S9Cfn from Fusobacterium nucleatum
Acta Crystallographica D Struct Biol 77 :1554

Wang X, Cheng MT, Chen ZP, Jiang YL, Ge YS, Xia R, Hou WT (2021)
Acta Crystallographica D Struct Biol 77 :1554