Wang_2025_Int.J.Biol.Macromol__141708

In the grain industry, lipases can hydrolyze the oils and fats in grains into glycerol and fatty acids, causing grain rancidity. If not detected and controlled, it would lead to the waste of grains and resources. In this research, rice bran was taken as the research object and a nanocomposite material with photoelectric activity, TiO(2)NFs-rGO, was developed by electrospinning technology. After immobilizing GDH enzyme, a photoelectrochemical biosensor, GDH-TiO(2)NFs-rGO-ITO, was constructed. When the glycerol content was within the range of 0.025-7 mmol/L, the photoelectric signal generated by the biosensor exhibited a favorable linear relationship with the glycerol content. The detection limit (S/N = 3) was 0.043 U/mL Even after seven uses, the detection of lipase activity still demonstrated high reproducibility. After being stored for 20 days, the photoelectric response value could still reach 81.37 % of that on the first day, indicating excellent storage stability. Moreover, other interfering substances in the test base solution did not generate obvious photoelectric responses, demonstrating high anti-interference ability. This biosensor indirectly, rapidly, and accurately detected lipase activity in rice bran. This study paves the way for the quality detection of rice bran during the storage period. In the future, it can be extended to the field of grain quality detection.