Title : Synthetic polyester-hydrolyzing enzymes from thermophilic actinomycetes - Wei_2014_Adv.Appl.Microbiol_89_267
Author(s) : Wei R , Oeser T , Zimmermann W
Ref : Adv Appl Microbiol , 89 :267 , 2014
Abstract :

Thermophilic actinomycetes produce enzymes capable of hydrolyzing synthetic polyesters such as polyethylene terephthalate (PET). In addition to carboxylesterases, which have hydrolytic activity predominantly against PET oligomers, esterases related to cutinases also hydrolyze synthetic polymers. The production of these enzymes by actinomycetes as well as their recombinant expression in heterologous hosts is described and their catalytic activity against polyester substrates is compared. Assays to analyze the enzymatic hydrolysis of synthetic polyesters are evaluated, and a kinetic model describing the enzymatic heterogeneous hydrolysis process is discussed. Structure-function and structure-stability relationships of actinomycete polyester hydrolases are compared based on molecular dynamics simulations and recently solved protein structures. In addition, recent progress in enhancing their activity and thermal stability by random or site-directed mutagenesis is presented.

PubMedSearch : Wei_2014_Adv.Appl.Microbiol_89_267
PubMedID: 25131405

Related information

Citations formats

Wei R, Oeser T, Zimmermann W (2014)
Synthetic polyester-hydrolyzing enzymes from thermophilic actinomycetes
Adv Appl Microbiol 89 :267

Wei R, Oeser T, Zimmermann W (2014)
Adv Appl Microbiol 89 :267