Title : Enzymatic Hydrolytic Resolution of Racemic Ibuprofen Ethyl Ester Using an Ionic Liquid as Cosolvent - Wei_2016_Molecules_21_ |
Author(s) : Wei T , Yang K , Bai B , Zang J , Yu X , Mao D |
Ref : Molecules , 21 : , 2016 |
Abstract :
The aim of this study was to develop an ionic liquid (IL) system for the enzymatic resolution of racemic ibuprofen ethyl ester to produce (S)-ibuprofen. Nineteen ILs were selected for use in buffer systems to investigate the effects of ILs as cosolvents for the production of (S)-ibuprofen using thermostable esterase (EST10) from Thermotoga maritima. Analysis of the catalytic efficiency and conformation of EST10 showed that [OmPy][BF(4)] was the best medium for the EST10-catalyzed production of (S)-ibuprofen. The maximum degree of conversion degree (47.4%), enantiomeric excess of (S)-ibuprofen (96.6%) and enantiomeric ratio of EST10 (177.0) were achieved with an EST10 concentration of 15 mg/mL, racemic ibuprofen ethyl ester concentration of 150 mM, at 75 degrees C , with a reaction time of 10 h. The reaction time needed to achieve the highest yield of (S)-ibuprofen was decreased from 24 h to 10 h. These results are relevant to the proposed application of ILs as solvents for the EST10-catalyzed production of (S)-ibuprofen. |
PubMedSearch : Wei_2016_Molecules_21_ |
PubMedID: 27420042 |
Substrate | Ibuprofen-Ethyl-Ester |
Wei T, Yang K, Bai B, Zang J, Yu X, Mao D (2016)
Enzymatic Hydrolytic Resolution of Racemic Ibuprofen Ethyl Ester Using an Ionic Liquid as Cosolvent
Molecules
21 :
Wei T, Yang K, Bai B, Zang J, Yu X, Mao D (2016)
Molecules
21 :