| Title : A Lab-in-a-Syringe Device Integrated with a Smartphone Platform: Colorimetric and Fluorescent Dual-Mode Signals for On-Site Detection of Organophosphorus Pesticides - Wei_2021_ACS.Appl.Mater.Interfaces__ |
| Author(s) : Wei D , Wang Y , Zhu N , Xiao J , Li X , Xu T , Hu X , Zhang Z , Yin D |
| Ref : ACS Appl Mater Interfaces , : , 2021 |
| Abstract : Herein, a portable lab-in-a-syringe device integrated with a smartphone sensing platform was designed for rapid, visual quantitative determination of organophosphorus pesticides (OPs) via colorimetric and fluorescent signals. The device was chiefly made up of a conjugate pad labeled with cetyltrimethylammonium bromide-coated gold nanoparticles (CTAB-Au NPs) and a sensing pad modified by ratiometric probes (red-emission quantum dots@SiO(2) nanoparticles@green-emission quantum dots, rQDs@SiO(2)@gQDs probe), which was assembled through a disposable syringe and reusable plastic filter. In the detection system, thiocholine (Tch), the hydrolysis product of thioacetylcholine (ATch) by acetylcholinesterase (AchE), could trigger the aggregation of CTAB-Au NPs, resulting in a significant color change from red to purple. Then, CTAB-Au NPs flowed vertically upward and bound to the rQDs@SiO(2)@gQDs probe on the sensing pad, reducing the fluorescence resonance energy transfer effect between CTAB-Au NPs and gQDs. Meanwhile, rQDs embedded in SiO(2) NPs remained stable as internal reference fluorescence, achieving a color transition from red to green. Thus, based on the inhibition of AChE activity by OPs, a colorimetric and fluorescent dual-mode platform was constructed for on-site detection of OPs. Using glyphosate as a model, with the support of a color recognizer application (APP) on a smartphone, the ratio of red and green channel values could be utilized for accurate OP quantitative analysis ranging from 0 to 10 microM with a detection limit of 2.81 nM (recoveries, 90.8-122.4%; CV, 1.2-3.4%). Overall, the portable lab-in-a-syringe device based on a smartphone sensing platform integrated sample monitoring and result analysis in the field, implying great potential for on-site detection of OPs. |
| ESTHER : Wei_2021_ACS.Appl.Mater.Interfaces__ |
| PubMedSearch : Wei_2021_ACS.Appl.Mater.Interfaces__ |
| PubMedID: 34623807 |
Wei D, Wang Y, Zhu N, Xiao J, Li X, Xu T, Hu X, Zhang Z, Yin D (2021)
A Lab-in-a-Syringe Device Integrated with a Smartphone Platform: Colorimetric and Fluorescent Dual-Mode Signals for On-Site Detection of Organophosphorus Pesticides
ACS Appl Mater Interfaces
:
Wei D, Wang Y, Zhu N, Xiao J, Li X, Xu T, Hu X, Zhang Z, Yin D (2021)
ACS Appl Mater Interfaces
: