Wei_2022_Chem.Pharm.Bull.(Tokyo)_70_283

A novel alkaloid caulophyine A (1) was isolated from the roots of Caulophyllum robustum Maxim., along with six known alkaloids 2-7. The structure of 1 was elucidated by extensive NMR and high resolution-time-of-flight (HR-TOF)-MS analyses, it is a rare nitrogen containing polycyclic aromatic hydrocarbon. The in vitro bioassays revealed that 2 presented remarkable cytotoxicity against A549 with an IC(50) value of 3.83 microM in comparison with the positive control etoposide (IC(50) = 11.63 microM). Compounds 1 and 2 also displayed weak Acetylcholinesterase (AChE) inhibitory activity with IC(50) values of 123.03 and 80.74 microM respectively.