Weigert_2021_Protein.Eng.Des.Sel_34_

Reference

Title : A versatile assay platform for enzymatic poly(ethylene-terephthalate) degradation - Weigert_2021_Protein.Eng.Des.Sel_34_
Author(s) : Weigert S , Gagsteiger A , Menzel T , Hocker B
Ref : Protein Engineering Des Sel , 34 : , 2021
Abstract :

Accumulation of plastic and subsequent microplastic is a major environmental challenge. With the discovery of potent polyethylene terephthalate (PET)-degrading enzymes, a new perspective arose for environmental decomposition as well as technical recycling. To explore the enormous diversity of potential PET-degrading enzymes in nature and also to conveniently employ techniques like protein engineering and directed evolution, a fast and reliable assay platform is needed. In this study we present our versatile solution applying a PET coating on standard lab consumables such as polymerase chain reaction tubes, 96- and 384-well microtiter plates, yielding an adjustable crystallinity of the PET. Combining the reaction vessels with either ultra-high performance liquid chromatography (UHPLC) or fluorometric readout and additional enzyme quantification offers a range of advantages. Thereby, the platform can easily be adapted to diverse needs from detailed analysis with high precision to high-throughput (HT) applications including crude lysate analysis.

PubMedSearch : Weigert_2021_Protein.Eng.Des.Sel_34_
PubMedID: 34427657

Related information

Citations formats

Weigert S, Gagsteiger A, Menzel T, Hocker B (2021)
A versatile assay platform for enzymatic poly(ethylene-terephthalate) degradation
Protein Engineering Des Sel 34 :

Weigert S, Gagsteiger A, Menzel T, Hocker B (2021)
Protein Engineering Des Sel 34 :