Weisstaub_2002_Hear.Res_167_122

The native cholinergic receptor that mediates synaptic transmission between olivocochlear fibers and outer hair cells of the cochlea is permeable to Ca(2+) and is thought to be composed of both the alpha 9 and the alpha 10 cholinergic nicotinic subunits. The aim of the present work was to study the permeability of the recombinant alpha 9 alpha 10 nicotinic acetylcholine receptor to Ca(2+), Ba(2+) and Mg(2+) and its modulation by these divalent cations. Experiments were performed, by the two-electrode voltage-clamp technique, in Xenopus laevis oocytes injected with alpha 9 and alpha 10 cRNA. The relative divalent to monovalent cation permeability was high ( approximately 10) for Ca(2+), Ba(2+) and Mg(2+). Currents evoked by acetylcholine (ACh) were potentiated by either Ca(2+) or Ba(2+) up to 500 microM but were blocked by higher concentrations of these cations. Potentiation by Ca(2+) was voltage-independent, whereas blockage was stronger at hyperpolarized than at depolarized potentials. Mg(2+) did not potentiate but it blocked ACh-evoked currents (IC(50)=0.38 mM). In the absence of Ca(2+), the EC(50) for ACh was higher (48 microM) than that obtained with 1.8 mM Ca(2+) (14.3 microM), suggesting that potentiation by Ca(2+) involves changes in the apparent affinity of the alpha 9 alpha 10 receptor for ACh.