Weng_1991_J.Gen.Microbiol_137_2539

Reference

Title : Purification, characterization and comparison of two non-haem bromoperoxidases from Streptomyces aureofaciens ATCC 10762 - Weng_1991_J.Gen.Microbiol_137_2539
Author(s) : Weng M , Pfeifer O , Krauss S , Lingens F , van Pee KH
Ref : J Gen Microbiol , 137 :2539 , 1991
Abstract :

Two non-haem bromoperoxidases (BPO 1 and BPO 2) were purified from the 7-chlorotetracycline-producing strain Streptomyces aureofaciens ATCC 10762. Both enzymes showed azide-insensitive brominating activity, and bromide-dependent peroxidase activity. BPO 1 was a dimer (Mr 65,000) with subunits of identical size (Mr 31,000). The pI was estimated to be 4.5. The enzyme did not cross-react with antibodies raised against the non-haem bromoperoxidase (Mr 90,000) from S. aureofaciens Tu24, a strain that also produces 7-chlorotetracycline. The Mr of BPO 2 was estimated to be 90,000. The enzyme had three identical subunits (Mr 31,000), and its isoelectric point was 3.5, identical with that of the bromoperoxidase from S. aureofaciens Tu24. Moreover, BPO 2 was immunologically identical with the bromoperoxidase from S. aureofaciens Tu24, although both it and BPO 1 could be distinguished electrophoretically from the latter bromoperoxidase.

PubMedSearch : Weng_1991_J.Gen.Microbiol_137_2539
PubMedID: 1783900
Gene_locus related to this paper: strau-brpa1 , strau-brpa2

Related information

Gene_locus strau-brpa1    strau-brpa2

Citations formats

Weng M, Pfeifer O, Krauss S, Lingens F, van Pee KH (1991)
Purification, characterization and comparison of two non-haem bromoperoxidases from Streptomyces aureofaciens ATCC 10762
J Gen Microbiol 137 :2539

Weng M, Pfeifer O, Krauss S, Lingens F, van Pee KH (1991)
J Gen Microbiol 137 :2539