| Title : Mammalian glial cells in culture synthesize acetylcholine - Wessler_1997_Naunyn.Schmiedebergs.Arch.Pharmacol_356_694 |
| Author(s) : Wessler I , Reinheimer T , Klapproth H , Schneider FJ , Racke K , Hammer R |
| Ref : Naunyn Schmiedebergs Arch Pharmacol , 356 :694 , 1997 |
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Abstract :
In the present study we demonstrate that acetylcholine is synthesized by cultured mammalian glial cells identified by cell-type specific markers. Primary cultures of rat brain astrocytes or microglia contained 2.0 and 1.6 pmol acetylcholine/10(6) cells on average respectively. Astrocyte cultures established from neonatal mouse brain contained even more acetylcholine (about 80 pmol acetylcholine/10(6) cells). Primary cultures of rat brain astrocytes showed choline acetyltransferase (ChAT) enzyme activity of 3 nmol/mg protein/h; ChAT activity was blocked by 10 microM bromoacetylcholine. In conclusion, these data demonstrate the synthesis of the "neurotransmitter" acetylcholine in cultured glial cells, a finding which opens a new view upon the role of acetylcholine in mammalian brain. |
| PubMedSearch : Wessler_1997_Naunyn.Schmiedebergs.Arch.Pharmacol_356_694 |
| PubMedID: 9402051 |
Wessler I, Reinheimer T, Klapproth H, Schneider FJ, Racke K, Hammer R (1997)
Mammalian glial cells in culture synthesize acetylcholine
Naunyn Schmiedebergs Arch Pharmacol
356 :694
Wessler I, Reinheimer T, Klapproth H, Schneider FJ, Racke K, Hammer R (1997)
Naunyn Schmiedebergs Arch Pharmacol
356 :694