| Title : Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain - Wiman_1982_Proc.Natl.Acad.Sci.U.S.A_79_1703 |
| Author(s) : Wiman K , Larhammar D , Claesson L , Gustafsson K , Schenning L , Bill P , Bohme J , Denaro M , Dobberstein B , Hammerling U , Kvist S , Servenius B , Sundelin J , Peterson PA , Rask L |
| Ref : Proc Natl Acad Sci U S A , 79 :1703 , 1982 |
|
Abstract :
The HLA-D locus in the major histocompatibility complex controls the expression of the genetically polymorphic HLA-DR antigens. mRNA coding for the beta chains of these antigens was partially purified from the human lymphoblastoid cell line Raji. The mRNA was copied into double-stranded cDNA and cloned in Escherichia coli. One clone, pDR-beta-1, obtained by hybrid selection, carries a 1070-base-pair insert comprising all of the coding region except the signal sequence and a substantial portion of the untranslated region. To identify pDR-beta-1, highly purified HLA-DR antigen beta chains derived from Raji cells were subjected to NH2-terminal amino acid sequence determination. This sequence displayed extensive homology with that deduced from the nucleotide sequence at the 5' end of the pDR-beta-1 coding region. Taken together, the amino acid and nucleotide sequences strongly argue in favor of Raji cells containing at least two beta-chain loci. |
| PubMedSearch : Wiman_1982_Proc.Natl.Acad.Sci.U.S.A_79_1703 |
| PubMedID: 6952222 |
Wiman K, Larhammar D, Claesson L, Gustafsson K, Schenning L, Bill P, Bohme J, Denaro M, Dobberstein B, Hammerling U, Kvist S, Servenius B, Sundelin J, Peterson PA, Rask L (1982)
Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain
Proc Natl Acad Sci U S A
79 :1703
Wiman K, Larhammar D, Claesson L, Gustafsson K, Schenning L, Bill P, Bohme J, Denaro M, Dobberstein B, Hammerling U, Kvist S, Servenius B, Sundelin J, Peterson PA, Rask L (1982)
Proc Natl Acad Sci U S A
79 :1703