Title : Cloning and high-level expression of a chloroperoxidase gene from Pseudomonas pyrrocinia in Escherichia coli - Wolfframm_1988_FEBS.Lett_238_325 |
Author(s) : Wolfframm C , van Pee KH , Lingens F |
Ref : FEBS Letters , 238 :325 , 1988 |
Abstract :
A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high-copy-number plasmid pUC18. In one subclone increased halogenating activity could be found which was 570-fold greater than in P. pyrrocinia. The halogenating enzyme was identified as the chloroperoxidase by SDS-polyacrylamide gel electrophoresis. |
PubMedSearch : Wolfframm_1988_FEBS.Lett_238_325 |
PubMedID: 3049160 |
Wolfframm C, van Pee KH, Lingens F (1988)
Cloning and high-level expression of a chloroperoxidase gene from Pseudomonas pyrrocinia in Escherichia coli
FEBS Letters
238 :325
Wolfframm C, van Pee KH, Lingens F (1988)
FEBS Letters
238 :325