Wolfframm_1993_Gene_130_131

Reference

Title : Chloroperoxidase-encoding gene from Pseudomonas pyrrocinia: sequence, expression in heterologous hosts, and purification of the enzyme - Wolfframm_1993_Gene_130_131
Author(s) : Wolfframm C , Lingens F , Mutzel R , van Pee KH
Ref : Gene , 130 :131 , 1993
Abstract :

The nucleotide sequence of a 1.5-kb fragment of Pseudomonas pyrrocinia DNA containing the chloroperoxidase(CPO)-encoding gene (cpo) and its flanking regions was determined. The cpo codes for a protein of 278 amino acids (aa). The mature enzyme contains no N-terminal methionine, so that the CPO monomer consists of 277 aa with a calculated M(r) of 30,304. Expression studies showed that the cpo from P. pyrrocinia is functionally expressed in Escherichia coli and Streptomyces lividans. Based on the overproduction of the CPO in E. coli, a novel and simple purification procedure was developed allowing the isolation of about 800-fold more CPO per gram of cells than was originally isolated from P. pyrrocinia. Comparison with the aa sequence of the bromoperoxidase BPO-A2 from S. aureofaciens ATCC10762 revealed an identity of 38%.

PubMedSearch : Wolfframm_1993_Gene_130_131
PubMedID: 8344520
Gene_locus related to this paper: psepy-prxc

Related information

Gene_locus psepy-prxc

Citations formats

Wolfframm C, Lingens F, Mutzel R, van Pee KH (1993)
Chloroperoxidase-encoding gene from Pseudomonas pyrrocinia: sequence, expression in heterologous hosts, and purification of the enzyme
Gene 130 :131

Wolfframm C, Lingens F, Mutzel R, van Pee KH (1993)
Gene 130 :131