Title : Synthesis of Short-Chain Alkyl Butyrate through Esterification Reaction Using Immobilized Rhodococcus Cutinase and Analysis of Substrate Specificity through Molecular Docking - Won_2022_J.Microbiol.Biotechnol_33_1 |
Author(s) : Won SJ , Yim JH , Kim HK |
Ref : J Microbiol Biotechnol , 33 :1 , 2022 |
Abstract :
Alkyl butyrate with fruity flavor is known as an important additive in the food industry. We synthesized various alkyl butyrates from various fatty alcohol and butyric acid using immobilized Rhodococcus cutinase (Rcut). Esterification reaction was performed in a non-aqueous system including heptane, isooctane, hexane, and cyclohexane. As a result of performing the alkyl butyrate synthesis reaction using alcohols of various chain lengths, it was found that the preference for the alcohol substrate had the following order: C6 > C4 > C8 > C10 > C2. Through molecular docking analysis, it was found that the greater the hydrophobicity of alcohol, the higher the accessibility to the active site of the enzyme. However, since the number of torsions increased as the chain length increased, it became difficult for the hydroxyl oxygen of the alcohol to access the gamma O of serine at the enzyme active site. These molecular docking results were consistent with substrate preference results of the Rcut enzyme. The Rcut maintained the synthesis efficiency at least for 5 days in isooctane solvent. We synthesized as much as 452 mM butyl butyrate by adding 100 mM substrate daily for 5 days and performing the reaction. These results show that Rcut is an efficient enzyme for producing alkyl butyrate used in the food industry. |
PubMedSearch : Won_2022_J.Microbiol.Biotechnol_33_1 |
PubMedID: 36524336 |
Gene_locus related to this paper: rhofa-a0a7i8e2z4 |
Substrate | Butyl-butyrate |
Gene_locus | rhofa-a0a7i8e2z4 |
Won SJ, Yim JH, Kim HK (2022)
Synthesis of Short-Chain Alkyl Butyrate through Esterification Reaction Using Immobilized Rhodococcus Cutinase and Analysis of Substrate Specificity through Molecular Docking
J Microbiol Biotechnol
33 :1
Won SJ, Yim JH, Kim HK (2022)
J Microbiol Biotechnol
33 :1