| Title : [Role of sialo compounds in choline uptake by synaptosomes] - Wong_1981_C.R.Seances.Acad.Sci.III_293_31 |
| Author(s) : Wong TY , Dreyfus H , Harth S , Louis JC , Mandel P , Massarelli R |
| Ref : Comptes Rendus de l Academie des Sciences , 293 :31 , 1981 |
|
Abstract :
Two kinetic mechanisms are responsible for the uptake of choline in Rat brain synaptosomal fractions with different affinity towards the substrate (high affinity mechanism: Km 5.1 microM, Vmax 44 pmol/mg prot/min.; low affinity mechanism: 35.7 microM, Vmax 286 pmol/mg prot/min). The treatment of synaptosomal fractions with neuraminidase (15 min. at 37 degrees C) followed by 1 min. incubation with various concentrations of [14C] choline reduces the uptake in parallel with the disappearance of the high affinity mechanism (Km 202.2 microM, Vmax 119 pmol/mg prot/min.), without influencing the synthesis of acetylcholine. |
| PubMedSearch : Wong_1981_C.R.Seances.Acad.Sci.III_293_31 |
| PubMedID: 6796200 |
Wong TY, Dreyfus H, Harth S, Louis JC, Mandel P, Massarelli R (1981)
[Role of sialo compounds in choline uptake by synaptosomes]
Comptes Rendus de l Academie des Sciences
293 :31
Wong TY, Dreyfus H, Harth S, Louis JC, Mandel P, Massarelli R (1981)
Comptes Rendus de l Academie des Sciences
293 :31