Wongwatanapaiboon_2016_Biosci.Biotechnol.Biochem_80_2231

cDNA of Aureobasidium melanogenum lipase comprises 1254 bp encoding 417 amino acids, whereas genomic DNA of lipase comprises 1311 bp with one intron (57 bp). The lipase gene contains a putative signal peptide encoding 26 amino acids. The A. melanogenum lipase gene was successfully expressed in Pichia pastoris. Recombinant lipase in an inducible expression system showed the highest lipase activity of 3.8 U/mL after six days of 2% v/v methanol induction. The molecular mass of purified recombinant lipase was estimated as 39 kDa using SDS-PAGE. Optimal lipase activity was observed at 35-37 degreesC and pH 7.0 using p-nitrophenyl laurate as the substrate. Lipase activity was enhanced by Mg(2+), Mn(2+), Li(+), Ca(2+), Ni(2+), CHAPS, DTT, and EDTA and inhibited by Hg(2+), Ag(+), SDS, Tween 20, and Triton X-100. The addition of 10% v/v acetone, DMSO, p-xylene, and octanol increased lipase activity, whereas that of propanol and butanol strongly inhibited it.