Woo_2007_Appl.Microbiol.Biotechnol_76_365

Reference

Title : Cloning and characterization of three epoxide hydrolases from a marine bacterium, Erythrobacter litoralis HTCC2594 - Woo_2007_Appl.Microbiol.Biotechnol_76_365
Author(s) : Woo JH , Hwang YO , Kang SG , Lee HS , Cho JC , Kim SJ
Ref : Applied Microbiology & Biotechnology , 76 :365 , 2007
Abstract :

Previously, we reported that ten strains belonging to Erythrobacter showed epoxide hydrolase (EHase) activities toward various epoxide substrates. Three genes encoding putative EHases were identified by analyzing open reading frames of Erythrobacter litoralis HTCC2594. Despite low similarities to reported EHases, the phylogenetic analysis of the three genes showed that eeh1 was similar to microsomal EHase, while eeh2 and eeh3 could be grouped with soluble EHases. The three EHase genes were cloned, and the recombinant proteins (rEEH1, rEEH2, and rEEH3) were purified. The functionality of purified proteins was proved by hydrolytic activities toward styrene oxide. EEH1 preferentially hydrolyzed (R)-styrene oxide, whereas EEH3 preferred to hydrolyze (S)-styrene oxide, representing enantioselective hydrolysis of styrene oxide. On the other hand, EEH2 could hydrolyze (R)- and (S)-styrene oxide at an equal rate. The optimal pH and temperature for the EHases occurred largely at neutral pHs and 40-55 degrees C. The substrate selectivity of rEEH1, rEEH2, and rEEH3 toward various epoxide substrates were also investigated. This is the first representation that a strict marine microorganism possessed three EHases with different enantioselectivity toward styrene oxide.

PubMedSearch : Woo_2007_Appl.Microbiol.Biotechnol_76_365
PubMedID: 17541582
Gene_locus related to this paper: 9sphn-q2n9r2 , 9sphn-q2n9t8 , 9sphn-q2nav3 , 9sphn-q2nch7

Related information

Gene_locus 9sphn-q2n9r2    9sphn-q2n9t8    9sphn-q2nav3    9sphn-q2nch7

Citations formats

Woo JH, Hwang YO, Kang SG, Lee HS, Cho JC, Kim SJ (2007)
Cloning and characterization of three epoxide hydrolases from a marine bacterium, Erythrobacter litoralis HTCC2594
Applied Microbiology & Biotechnology 76 :365

Woo JH, Hwang YO, Kang SG, Lee HS, Cho JC, Kim SJ (2007)
Applied Microbiology & Biotechnology 76 :365