| Title : Acetylcholine synthesis and accumulation in the CNS of Drosophila larvae: analysis of shibirets, a mutant with a temperature-sensitive block in synaptic transmission - Wu_1983_J.Neurochem_40_1386 |
| Author(s) : Wu CF , Berneking JM , Barker DL |
| Ref : Journal of Neurochemistry , 40 :1386 , 1983 |
|
Abstract :
A radiochemical method is applied to the study of neurotransmitter metabolism in Drosophila. The larval CNS is a favorable system for analyzing acetylcholine (ACh) metabolism, since the pool of [3H]ACh rapidly reaches a steady state with a high ratio of intracellular [3H]ACh to [3H]choline. A temperature-sensitive paralytic mutant, shibirets, shows reduced [3H]ACh accumulation at the restrictive temperature. This reduction is not the result of decreased synthesis of [3H]ACh, but rather an abnormally rapid rate of release, which is not prevented by blocking tetrodotoxin-sensitive nerve activity. |
| PubMedSearch : Wu_1983_J.Neurochem_40_1386 |
| PubMedID: 6300334 |
Wu CF, Berneking JM, Barker DL (1983)
Acetylcholine synthesis and accumulation in the CNS of Drosophila larvae: analysis of shibirets, a mutant with a temperature-sensitive block in synaptic transmission
Journal of Neurochemistry
40 :1386
Wu CF, Berneking JM, Barker DL (1983)
Journal of Neurochemistry
40 :1386