Wu_2010_J.Exp.Bot_61_3407

Reference

Title : Isolation and characterization of low-sulphur-tolerant mutants of Arabidopsis - Wu_2010_J.Exp.Bot_61_3407
Author(s) : Wu Y , Zhao Q , Gao L , Yu XM , Fang P , Oliver DJ , Xiang CB
Ref : J Exp Bot , 61 :3407 , 2010
Abstract :

Sulphur is an essential element for plant growth and development as well as for defence against biotic and abiotic stresses. Increasing sulphate utilization efficiency (SUE) is an important issue for crop improvement. Little is known about the genetic determinants of sulphate utilization efficiency. No gain-of-function mutants with improved SUE have been reported to date. Here the isolation and characterization of two low-sulphur-tolerant mutants, sue3 and sue4 are reported using a high-throughput genetic screen where a 'sulphur-free' solid medium was devised to give the selection pressure necessary to suppress the growth of the wild-type seedlings. Both mutants showed improved tolerance to low sulphur conditions and well-developed root systems. The mutant phenotype of both sue3 and sue4 was specific to sulphate deficiency and the mutants displayed enhanced tolerance to heavy metal and oxidative stress. Genetic analysis revealed that sue3 was caused by a single recessive nuclear mutation while sue4 was caused by a single dominant nuclear mutation. The recessive locus in sue3 is the previously identified VirE2-interacting Protein 1. The dominant locus in sue4 is a function-unknown locus activated by the four enhancers on the T-DNA. The function of SUE3 and SUE4 in low sulphur tolerance was confirmed either by multiple mutant alleles or by recapitulation analysis. Taken together, our results demonstrate that this genetic screen is a reasonable approach to isolate Arabidopsis mutants with improved low sulphur tolerance and potentially with enhanced sulphate utilization efficiency. The two loci identified in sue3 and sue4 should assist in understanding the molecular mechanisms of low sulphur tolerance.

PubMedSearch : Wu_2010_J.Exp.Bot_61_3407
PubMedID: 20547563

Related information

Gene_locus_frgt arath-f4iy46

Citations formats

Wu Y, Zhao Q, Gao L, Yu XM, Fang P, Oliver DJ, Xiang CB (2010)
Isolation and characterization of low-sulphur-tolerant mutants of Arabidopsis
J Exp Bot 61 :3407

Wu Y, Zhao Q, Gao L, Yu XM, Fang P, Oliver DJ, Xiang CB (2010)
J Exp Bot 61 :3407