| Title : Characterization of QmnD3\/QmnD4 for double bond formation in quartromicin biosynthesis - Wu_2014_Org.Lett_16_1578 |
| Author(s) : Wu LF , He HY , Pan HX , Han L , Wang R , Tang GL |
| Ref : Org Lett , 16 :1578 , 2014 |
|
Abstract :
In this work, two enzymes responsible for the biogenesis of possible [4 + 2] reaction precursors in the quartromicin biosynthetic pathway were characterized: acetylation of 1 to yield 2 was catalyzed by QmnD3, and subsequent acetic acid elimination of 2 to form double bond product 3 was catalyzed by QmnD4. Site-directed mutagenesis assay of QmnD3 and QmnD4 was investigated, and a general base-catalyzed mechanism for QmnD4 is proposed. |
| PubMedSearch : Wu_2014_Org.Lett_16_1578 |
| PubMedID: 24580034 |
Wu LF, He HY, Pan HX, Han L, Wang R, Tang GL (2014)
Characterization of QmnD3\/QmnD4 for double bond formation in quartromicin biosynthesis
Org Lett
16 :1578
Wu LF, He HY, Pan HX, Han L, Wang R, Tang GL (2014)
Org Lett
16 :1578