Title : Role of non-neuronal cholinergic system in the early stage response of epithelial-mesenchymal transformation related markers in A549 cells induced by coal particles - Wu_2022_Heliyon_8_e11751 |
Author(s) : Wu MY , Shi XC , Shan J , Wang R , Wang Y , Li J , Tian DN , Xu HM |
Ref : Heliyon , 8 :e11751 , 2022 |
Abstract :
OBJECTIVE: This study was aimed to investigate the role of non-neuronal cholinergic system (NNCS) in the early stage response of epithelial-mesenchymal transformation (EMT) related markers in human lung adenocarcinoma A549 cells induced by coal particles. METHODS: A549 cells were exposed to different concentrations of GBW11110K, GBW11126D and exogenous acetylcholinesterase (AChE) (the exposure doses were determined according to the results of CCK-8 experiment, and the doses that had no significant effects on cell viability were selected) for 24 h. After exposure, the indexes of oxidative stress (SOD and MDA), inflammatory factors (IL-6 and TNF-alpha), EMT marker proteins (E-cadherin and vimentin), AChE enzymatic activity and mRNA expression levels of different types of acetylcholine receptors (CHRM3, CHRM5, CHRNA5, CHRNA7, CHRNA9 and CHRNB2) were determined. RESULTS: GBW11110K and GBW11126D exposure could lead to the following injury effects: the levels of oxidative stress and inflammatory factors changed to a certain extent (SOD decreased gradually, while MDA, IL-6 and TNF-alpha increased). The protein level of E-cadherin decreased while the vimentin level increased (P < 0.05), suggesting the occurrence of EMT. The AChE enzymatic activity decreased gradually. The expression of acetylcholine receptor mRNA changed as follows (GBW11110K/GBW11126D: CHRM3 (++), CHRM5 (++), CHRNA5 (++), CHRNA7 (++), CHRNA9 (- +), CHRNB2 (- -). The addition of exogenous AChE recombinant protein could antagonize the damage effects caused by the coal particles to a certain extent. CONCLUSION: The coal particle exposure could induce the change of oxidative stress response, inflammatory response and EMT related markers, down-regulate the AChE enzymatic activity, and interfere the mRNA expression levels of AChRs in A549 cells. The addition of exogenous AChE recombinant protein could reverse the above effects to a certain extent. |
PubMedSearch : Wu_2022_Heliyon_8_e11751 |
PubMedID: 36468138 |
Wu MY, Shi XC, Shan J, Wang R, Wang Y, Li J, Tian DN, Xu HM (2022)
Role of non-neuronal cholinergic system in the early stage response of epithelial-mesenchymal transformation related markers in A549 cells induced by coal particles
Heliyon
8 :e11751
Wu MY, Shi XC, Shan J, Wang R, Wang Y, Li J, Tian DN, Xu HM (2022)
Heliyon
8 :e11751