Wu_2025_Nat.Prod.Res__1

Phytochemical investigation on the aerial part of Fritillaria walujewii Regel resulted in the isolation of two new steroidal alkaloids, named as walujewine F (1) and G (2), and five previously reported compounds 3-7. Structural elucidation of compounds 1-7 was achieved by employing various spectroscopic techniques. The cholinesterase inhibitory potential of all the compounds was systematically evaluated. Compound 1 exhibited moderate AChE inhibition with the IC(50) value of 70.37 microM, while compound 2 demonstrated obvious dual AChE and BChE inhibitory effects, with the IC(50) values of 91.69 microM and 67.12 microM, respectively, but lower than the positive control galantamine (IC(50) values of 2.80 microM for AChE and 21.85 microM for BChE).