| Title : A Novel Paper-Based Electrochemical Biosensor Based on N,O-Rich Covalent Organic Frameworks for Carbaryl Detection - Xiao_2022_Biosensors.(Basel)_12_ |
| Author(s) : Xiao Y , Wu N , Wang L , Chen L |
| Ref : Biosensors (Basel) , 12 : , 2022 |
| Abstract : A new N,O-rich covalent organic framework (COF(DHNDA-BTH)) was synthesized by an amine-aldehyde condensation reaction between 2,6-dialdehyde-1,5-dihydroxynaphthalene (DHNDA) and 1,3,5-phenyltriformylhydrazine (BTH) for carbaryl detection. The free NH, OH, and C=O groups of COF(DHNDA-BTH) not only covalently couples with acetylcholinesterase (AChE) into the pores of COF(DHNDA-BTH), but also greatly improves the catalytic activity of AChE in the constrained environment of COF(DHNDA-BTH)'s pore. Under the catalysis of AChE, the acetylthiocholine (ATCl) was decomposed into positively charged thiocholine (TCl), which was captured on the COF(DHNDA-BTH) modified electrode. The positive charges of TCl can attract anionic probe [Fe(CN)(6)](3-/4-) on the COF(DHNDA-BTH)-modified electrode to show a good oxidation peak at 0.25 V (versus a saturated calomel electrode). The carbaryl detection can inhibit the activity of AChE, resulting in the decrease in the oxidation peak. Therefore, a turn-off electrochemical carbaryl biosensor based on a flexible carbon paper electrode loaded with COF(DHNDA-BTH) and AChE was constructed using the oxidation peak of an anionic probe [Fe(CN)(6)](3-/4-) as the detection signal. The detection limit was 0.16 microM (S/N = 3), and the linear range was 0.48~35.0 microM. The sensor has good selectivity, repeatability, and stability, and has a good application prospect in pesticide detection. |
| ESTHER : Xiao_2022_Biosensors.(Basel)_12_ |
| PubMedSearch : Xiao_2022_Biosensors.(Basel)_12_ |
| PubMedID: 36291036 |
Xiao Y, Wu N, Wang L, Chen L (2022)
A Novel Paper-Based Electrochemical Biosensor Based on N,O-Rich Covalent Organic Frameworks for Carbaryl Detection
Biosensors (Basel)
12 :
Xiao Y, Wu N, Wang L, Chen L (2022)
Biosensors (Basel)
12 :