Xie_2016_Evid.Based.Complement.Alternat.Med_2016_7481323

Reference

Title : Primary Investigation for the Mechanism of Biatractylolide from Atractylodis Macrocephalae Rhizoma as an Acetylcholinesterase Inhibitor - Xie_2016_Evid.Based.Complement.Alternat.Med_2016_7481323
Author(s) : Xie YC , Ning N , Zhu L , Li DN , Feng X , Yang XP
Ref : Evid Based Complement Alternat Med , 2016 :7481323 , 2016
Abstract :

Biatractylolide was isolated from ethyl acetate extract of dried Atractylodis Macrocephalae Rhizoma root by multistep chromatographic processing. Structure of biatractylolide was confirmed by (1)H-NMR and (13)C-NMR. The IC50 on acetylcholinesterase (AChE) activity was 6.5458 mug/mL when the control IC50 value of huperzine A was 0.0192 mug/mL. Molecular Docking Software (MOE) was used to discover molecular sites of action between biatractylolide and AChE protein by regular molecular docking approaches. Moreover, biatractylolide downregulated the expression of AChE of MEF and 293T cells in a dose-dependent manner. These results demonstrated that the molecular mechanisms of inhibitory activities of biatractylolide on AChE are not only through binding to AChE, but also via reducing AChE expression by inhibiting the activity of GSK3beta.

PubMedSearch : Xie_2016_Evid.Based.Complement.Alternat.Med_2016_7481323
PubMedID: 27642355

Related information

Citations formats

Xie YC, Ning N, Zhu L, Li DN, Feng X, Yang XP (2016)
Primary Investigation for the Mechanism of Biatractylolide from Atractylodis Macrocephalae Rhizoma as an Acetylcholinesterase Inhibitor
Evid Based Complement Alternat Med 2016 :7481323

Xie YC, Ning N, Zhu L, Li DN, Feng X, Yang XP (2016)
Evid Based Complement Alternat Med 2016 :7481323