Yang_2005_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_61_703

Reference

Title : Cloning purification crystallisation and prliminary X-ray characterisation of a conserved hypothetical protein XC6422 from Xanthomonas campestris - Yang_2005_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_61_703
Author(s) : Yang CY , Chin KH , Shr HL , Gao FP , Lyu PC , Wang AHJ
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , F61 :703 , 2005
Abstract :

Xanthomonas campestris pv. campestris is a Gram-negative yellow-pigmented pathogenic bacterium that causes black rot, one of the major worldwide diseases of cruciferous crops. Its genome contains approximately 4500 genes, roughly one third of which have no known structure and/or function. However, some genes of unknown function are highly conserved among several different bacterial genuses. XC6422 is one such conserved hypothetical protein and has been overexpressed in Escherichia coli, purified and crystallized in a variety of forms using the hanging-drop vapour-diffusion method. Crystals grew to approximately 2 x 1.5 x 0.4 mm in size after one week and diffracted to at least 1.6 A resolution. They belong to the monoclinic space group C2, with one molecule per asymmetric unit and unit-cell parameters a = 75.8, b = 79.3, c = 38.2 A, beta = 109.4 degrees. Determination of this structure may provide insights into the protein's function.

PubMedSearch : Yang_2005_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_61_703
PubMedID: 16511134
Gene_locus related to this paper: xanca-XC6422

Related information

Gene_locus xanca-XC6422
Structure 2FUK

Citations formats

Yang CY, Chin KH, Shr HL, Gao FP, Lyu PC, Wang AHJ (2005)
Cloning purification crystallisation and prliminary X-ray characterisation of a conserved hypothetical protein XC6422 from Xanthomonas campestris
Acta Crystallographica Sect F Struct Biol Cryst Commun F61 :703

Yang CY, Chin KH, Shr HL, Gao FP, Lyu PC, Wang AHJ (2005)
Acta Crystallographica Sect F Struct Biol Cryst Commun F61 :703