| Title : Monoclonal antibodies to the thyrotropin receptor: implications for receptor structure and the action of autoantibodies in Graves disease - Yavin_1981_Proc.Natl.Acad.Sci.U.S.A_78_3180 |
| Author(s) : Yavin E , Yavin Z , Schneider MD , Kohn LD |
| Ref : Proc Natl Acad Sci U S A , 78 :3180 , 1981 |
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Abstract :
Hybridoma cells have been obtained by fusing P3-NS1/1-Ag4-1 mouse myeloma cells with spleen cells from mice immunized with solubilized preparations of the thyrotropin receptor. Five clones were produced that secrete a monoclonal antibody whose binding to thyroid membranes is specifically inhibited by unlabeled thyrotropin. The antibody interacts with functioning thyroid cells in culture but not with nonfunctioning cells; this interaction is prevented by thyrotropin. The antibodies are capable of competitively blocking thyrotropin binding to bovine thyroid membrane preparations; they prevent 125I-labeled thyrotropin binding to a solubilized preparation of the glycoprotein component of the bovine thyrotropin receptor but are unable to inhibit 125I-labeled thyrotropin binding to liposomes containing gangliosides at comparable concentrations. They prevent 125I-labeled thyrotropin binding to rat, bovine, or human (Graves disease) thyroid membrane preparations. They do not stimulate adenylate cyclase activity in thyroid membrane preparations but can inhibit thyrotropin-stimulated iodide uptake by functioning thyroid cells in culture. |
| PubMedSearch : Yavin_1981_Proc.Natl.Acad.Sci.U.S.A_78_3180 |
| PubMedID: 6265939 |
Yavin E, Yavin Z, Schneider MD, Kohn LD (1981)
Monoclonal antibodies to the thyrotropin receptor: implications for receptor structure and the action of autoantibodies in Graves disease
Proc Natl Acad Sci U S A
78 :3180
Yavin E, Yavin Z, Schneider MD, Kohn LD (1981)
Proc Natl Acad Sci U S A
78 :3180