Ye_2015_Biosens.Bioelectron_79_34

Reference

Title : Highly sensitive electrochemiluminescenc assay of acetylcholinesterase activity based on dual biomarkers using Pd-Au nanowires as immobilization platform - Ye_2015_Biosens.Bioelectron_79_34
Author(s) : Ye C , Wang MQ , Zhong X , Chen S , Chai Y , Yuan R
Ref : Biosensors & Bioelectronics , 79 :34 , 2015
Abstract :

One-dimensional Pd-Au nanowires (Pd-Au NWs) were prepared and applied to fabricate an electrochemiluminescence (ECL) biosensor for the detection of acetylcholinesterase (AChE) activity. Compared with single-component of Pd or Au, the bimetallic nanocomposite of Pd-Au NWs offers a larger surface area for the immobilization of enzyme, and displays superior electrocatalytic activity and efficient electron transport capacity. In the presence of AChE and choline oxidase (ChOx), acetylcholine (ATCl) is hydrolyzed by AChE to generate thiocholine, then thiocholine is catalyzed by ChOx to produce H2O2in situ, which serves as the coreactant to effectively enhance the ECL intensity in luminol-ECL system. The detection principle is based on the inhibited AChE and reactivated AChE as dual biomarkers, in which AChE was inhibited by organophosphorus (OP) agents, and then reactivated by obidoxime. Such dual biomarkers method can achieve credible evaluation for AChE activity via providing AChE activity before and after reactivation. The liner range for AChE activity detection was from 0.025UL-1 to 25KUL-1 with a low detection limit down to 0.0083UL-1.

PubMedSearch : Ye_2015_Biosens.Bioelectron_79_34
PubMedID: 26686921

Related information

Citations formats

Ye C, Wang MQ, Zhong X, Chen S, Chai Y, Yuan R (2015)
Highly sensitive electrochemiluminescenc assay of acetylcholinesterase activity based on dual biomarkers using Pd-Au nanowires as immobilization platform
Biosensors & Bioelectronics 79 :34

Ye C, Wang MQ, Zhong X, Chen S, Chai Y, Yuan R (2015)
Biosensors & Bioelectronics 79 :34