Yin_2016_Catal.Sci.Technol_6_6305

A novel non-heme chloroperoxidase (SvGL) with promiscuous (-)-gamma-lactamase activity towards Vince lactam was identified from Streptomyces viridochromogenes by genome data-mining. SvGL possesses high activity and excellent thermal stability and enantioselectivity. Furthermore, it is able to tolerate extremely high substrate concentrations (4.0 M, 436.5 g L-1). Using the newly discovered (-)-gamma-lactamase as a biocatalyst, an efficient and environmentally benign process for the production of (+)-gamma-lactam was developed. The process allowed an enantioselective resolution of 436.5 g L-1 racemic gamma-lactam with only 0.2 g L-1 lyophilized cell-free extract, affording an extremely high substrate/catalyst ratio of 2183 (g g-1), a space-time yield of 458 g L-1 d-1, and a very low E factor (environmental factor) of 5.7 (kg waste per kg product) even when the process water is included.