| Title : Inhibition by neuroprotective drug NS-7 of nicotine-induced 22Na(+) influx, 45Ca(2+) influx and catecholamine secretion in adrenal chromaffin cells - Yokoo_2000_Brain.Res_873_149 |
| Author(s) : Yokoo H , Shiraishi S , Kobayashi H , Yanagita T , Minami S , Yamamoto R , Wada A |
| Ref : Brain Research , 873 :149 , 2000 |
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Abstract :
In cultured bovine adrenal chromaffin cells, NS-7 [4-(4-fluorophenyl)-2-methyl-6-(5-piperidinopentyloxy) pyrimidine hydrochloride], a newly-synthesized neuroprotective drug, inhibited nicotine-induced 22Na(+) influx via nicotinic receptors (IC(50)=15.5 microM); the suppression by NS-7 was observed in the presence of ouabain, an inhibitor of Na(+),K(+)-ATPase, and was not attenuated upon the washout of NS-7. NS-7 decreased nicotine-induced maximum influx of 22Na(+) without altering the EC(50) value of nicotine. Also, NS-7 diminished nicotine-induced 45Ca(2+) influx via nicotinic receptors and voltage-dependent Ca(2+) channels (IC(50)=14.1 microM) and catecholamine secretion (IC(50)=19.5 microM). These results suggest that NS-7 produces noncompetitive and long-lasting inhibitory effects on neuronal nicotinic receptors in adrenal chromaffin cells, and interferes with the stimulus-secretion coupling. |
| PubMedSearch : Yokoo_2000_Brain.Res_873_149 |
| PubMedID: 10915823 |
Yokoo H, Shiraishi S, Kobayashi H, Yanagita T, Minami S, Yamamoto R, Wada A (2000)
Inhibition by neuroprotective drug NS-7 of nicotine-induced 22Na(+) influx, 45Ca(2+) influx and catecholamine secretion in adrenal chromaffin cells
Brain Research
873 :149
Yokoo H, Shiraishi S, Kobayashi H, Yanagita T, Minami S, Yamamoto R, Wada A (2000)
Brain Research
873 :149