Yoneyama_2020_Plant.Direct_4_e00219

Reference

Title : Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis - Yoneyama_2020_Plant.Direct_4_e00219
Author(s) : Yoneyama K , Akiyama K , Brewer PB , Mori N , Kawano-Kawada M , Haruta S , Nishiwaki H , Yamauchi S , Xie X , Umehara M , Beveridge CA , Nomura T
Ref : Plant Direct , 4 :e00219 , 2020
Abstract :

Strigolactones (SLs) regulate important aspects of plant growth and stress responses. Many diverse types of SL occur in plants, but a complete picture of biosynthesis remains unclear. In Arabidopsis thaliana, we have demonstrated that MAX1, a cytochrome P450 monooxygenase, converts carlactone (CL) into carlactonoic acid (CLA) and that LBO, a 2-oxoglutarate-dependent dioxygenase, can convert methyl carlactonoate (MeCLA) into a metabolite called [MeCLA + 16 Da]. In the present study, feeding experiments with deuterated MeCLAs revealed that [MeCLA + 16 Da] is hydroxymethyl carlactonoate (1'-HO-MeCLA). Importantly, this LBO metabolite was detected in plants. Interestingly, other related compounds, methyl 4-hydroxycarlactonoate (4-HO-MeCLA) and methyl 16-hydroxycarlactonoate (16-HO-MeCLA), were also found to accumulate in lbo mutants. 3-HO-, 4-HO-, and 16-HO-CL were detected in plants, but their expected corresponding metabolites, HO-CLAs, were absent in max1 mutants. These results suggest that HO-CL derivatives may be predominant SLs in Arabidopsis, produced through MAX1 and LBO.

PubMedSearch : Yoneyama_2020_Plant.Direct_4_e00219
PubMedID: 32399509

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Citations formats

Yoneyama K, Akiyama K, Brewer PB, Mori N, Kawano-Kawada M, Haruta S, Nishiwaki H, Yamauchi S, Xie X, Umehara M, Beveridge CA, Nomura T (2020)
Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis
Plant Direct 4 :e00219

Yoneyama K, Akiyama K, Brewer PB, Mori N, Kawano-Kawada M, Haruta S, Nishiwaki H, Yamauchi S, Xie X, Umehara M, Beveridge CA, Nomura T (2020)
Plant Direct 4 :e00219