| Title : Enantioselective resolution of racemic styrene oxide at high concentration using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis in the presence of surfactant and glycerol - Yoo_2008_Biotechnol.Lett_30_1807 |
| Author(s) : Yoo SS , Park S , Lee EY |
| Ref : Biotechnol Lett , 30 :1807 , 2008 |
|
Abstract :
The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatalyst. Enantiopure (S)-styrene oxide with 98% ee was obtained with 41% yield (maximum yield = 50%) from 1.8 M racemic styrene oxide at pH 8.0, 4 degrees C in the presence of 40% (v/v) Tween 20 and 5% (v/v) glycerol. |
| PubMedSearch : Yoo_2008_Biotechnol.Lett_30_1807 |
| PubMedID: 18575813 |
| Gene_locus related to this paper: rhogl-EPH1 |
| Substrate | Styrene-oxide |
| Gene_locus | rhogl-EPH1 |
Yoo SS, Park S, Lee EY (2008)
Enantioselective resolution of racemic styrene oxide at high concentration using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis in the presence of surfactant and glycerol
Biotechnol Lett
30 :1807
Yoo SS, Park S, Lee EY (2008)
Biotechnol Lett
30 :1807