Yoshimaru_2021_Pediatr.Int__

Reference

Title : Reevaluation of Concurrent AChE and HE staining for Hirschsprung's disease - Yoshimaru_2021_Pediatr.Int__
Author(s) : Yoshimaru K , Matsuura T , Yanagi Y , Obata S , Takahashi Y , Kajihara K , Ohmori A , Irie K , Hino Y , Shibui Y , Tamaki A , Kohashi K , Oda Y , Taguchi T
Ref : Pediatr Int , : , 2021
Abstract :

BACKGROUND: Acetylcholinesterase (AChE) histochemistry has been widely performed for the histopathological diagnosis of Hirschsprung's disease (HD). However, we occasionally come across diagnostic difficulties. We conducted concurrent AChE histochemistry and hematoxylin and eosin (HE) staining to validate the ancillary value of this technique. METHODS: Of 177 patients diagnosed using AChE histochemistry from January 2014 to December 2016, 90 patients underwent formalin-fixed paraffin-embedded HE staining. The histopathological findings and diagnostic abilities were retrospectively investigated and compared. RESULTS: The sensitivity, specificity, accuracy and Kappa index of AChE histochemistry and HE staining were 94.1%, 100%, 98.9% and 0.964 and 76.5%, 84.9%, 83.3% and 0.530, respectively, with the specificity, accuracy and Kappa index of AChE histochemistry significantly higher than those of HE staining (p<0.001, p<0.001 and p<0.05). HE staining supported the suspected diagnosis of total colon aganglionosis (TCA) at the initial biopsy; furthermore, HE staining helped confirm the distinct shape of ganglion cells and hypertrophic nerve bundles. CONCLUSION: We re-confirmed that AChE histochemistry is an excellent method for diagnosing HD. Although the diagnostic ability of HE staining is limited, it has acceptable utility as an ancillary method. Thus, AChE staining is a useful test and it should be performed together with HE staining.

PubMedSearch : Yoshimaru_2021_Pediatr.Int__
PubMedID: 33417724

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Citations formats

Yoshimaru K, Matsuura T, Yanagi Y, Obata S, Takahashi Y, Kajihara K, Ohmori A, Irie K, Hino Y, Shibui Y, Tamaki A, Kohashi K, Oda Y, Taguchi T (2021)
Reevaluation of Concurrent AChE and HE staining for Hirschsprung's disease
Pediatr Int :

Yoshimaru K, Matsuura T, Yanagi Y, Obata S, Takahashi Y, Kajihara K, Ohmori A, Irie K, Hino Y, Shibui Y, Tamaki A, Kohashi K, Oda Y, Taguchi T (2021)
Pediatr Int :