Yuan_2024_Pestic.Biochem.Physiol_203_105996

Reference

Title : Swift regulation of nicotinic acetylcholine receptors (nAChRs) and glutathione S-transferase (GST) enables the rapid detoxification of thiacloprid in pine sawyer beetles - Yuan_2024_Pestic.Biochem.Physiol_203_105996
Author(s) : Yuan H , Wu M , Deng J , Zhou M , Wickham JD , Zhang L
Ref : Pestic Biochem Physiol , 203 :105996 , 2024
Abstract :

Thiacloprid, a neonicotinoid insecticide, has become one of the major control agents for the pine sawyer beetle, Monochamus alternatus Hope, however, the mechanism of detoxification is unknown. We demonstrate that glutathione S-transferases (GSTs) and nicotinic acetylcholine receptors (nAChRs) are involved in the rapid detoxification of thiacloprid in M. alternatus larvae. The activity of detoxification enzyme GSTs was significantly higher, while the activity of acetylcholinesterase (AChE) was inhibited under thiacloprid exposure. The inhibition of AChE activity led to lethal over-stimulation of the cholinergic synapse, which was then released by the rapid downregulation of nAChRs. Meanwhile, GSTs were overexpressed to detoxify thiacloprid accordingly. A total of 3 nAChR and 12 GST genes were identified from M. alternatus, among which ManAChRalpha2 and MaGSTs1 were predicted to confer thiacloprid tolerance. RNA interference (RNAi) was subsequently conducted to confirm the function of ManAChRalpha2 and MaGSTs1 genes in thiacloprid detoxification. The successful knock-down of the ManAChRalpha2 gene led to lower mortality of M. alternatus under LC(30) thiacloprid treatment, and the suppression of the MaGSTs1 gene increased the mortality rate of M. alternatus. However, the mortality rate has no significant difference with controls when thiacloprid was fed together with both dsMaGSTs1 and dsManAChRalpha2. Molecular docking modeled the molecular basis for interaction between MaGSTs1/ManAChR and thiacloprid. This study highlights the important roles that ManAChRalpha2 and MaGSTs1 genes play in thiacloprid detoxification through transcriptional regulation and enzymatic metabolization, and proposes a new avenue for integrated pest management that combines pesticides and RNAi technology as an efficient strategy for M. alternatus control.

PubMedSearch : Yuan_2024_Pestic.Biochem.Physiol_203_105996
PubMedID: 39084770

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Citations formats

Yuan H, Wu M, Deng J, Zhou M, Wickham JD, Zhang L (2024)
Swift regulation of nicotinic acetylcholine receptors (nAChRs) and glutathione S-transferase (GST) enables the rapid detoxification of thiacloprid in pine sawyer beetles
Pestic Biochem Physiol 203 :105996

Yuan H, Wu M, Deng J, Zhou M, Wickham JD, Zhang L (2024)
Pestic Biochem Physiol 203 :105996