Zahoor_2021_Heliyon_7_e06367

Medicines derived from plants are preferred over synthetic therapeutic agents in treating different diseases. Ziziphus oxyphylla (a member of Rhamnaceae family) is a medicinal plant used as a remedy of different diseases in Greek and Ayurveda medical systems. Z. oxyphylla roots were shade dried and then subjected to extraction of bioactive compounds using different solvent systems and silica gel. From ethyl acetate fraction, three compounds viz., p-coumaric acid (V), 3,4-dimethoxy benzoic acid (VI), and 4-heptyloxy benzoic acid (VII) were isolated in pure form. The selection of ethyl acetate fraction for isolation was based on HPLC profiling of crude extract and different fractions. These compounds were characterized by different spectroscopic techniques and evaluated for their in vitro antioxidant, anticholinesterase, alpha-glucosidase, and alpha-amylase inhibitory potentials. To find out possible binding interactions of V with AChE and BChE crystals, in-silico docking studies were also carried out. Compound V showed maximum scavenging capabilities of DPPH and ABTS free radicals with IC(50) values of 69 and 62 microg/mL respectively. Excellent percent inhibition (83.4 +/- 0.5% at highest concentration 1000 microg/mL) of acetylcholinesterase (AChE) was exhibited by compound V (IC(50) = 80 microg/mL); whereas, for the mentioned concentration, 83.2 +/- 1.1% inhibition (IC(50) = 90 microg/mL) of butyrylcholinesterase (BChE) was observed as well. The compound VI exhibited highest % inhibition against alpha-glucosidase (IC(50) = 84 microg/mL) whereas alpha-amylase was more potently inhibited by compound V (% inhibition = 86.8 % and IC(50) = 85 microg/mL). Docking scores of -1.391 Kcal/mol (BChE) and -6.253 Kcal/mol (AChE) were recorded using molecular docking software. Compound V exhibited strong free radical scavenging and anticholinesterase potentials suggesting that it can be effectively used to treat oxidative stress and dementia in human.